Figure 8
From: Regulation of multiple tip formation by caffeine in cellular slime molds
Cell sorting during secondary tip formation. The ecmA expressing part of slug (AX2-ecmA-GFP) was removed using a coverslip under an epi-fluorescent stereo zoom microscope. ecmA expression in control slugs (non-nutrient agar plate without caffeine) and those in caffeine containing plates was monitored at indicated time points. The arrow shows ecmA-GFP expression in the prespore region in prestalk ablated slug. Scale bar = 200 μm.