Figure 7

Cell movement in slugs during secondary tip formation. AX2 cells were labeled with a fluorescent dye (9 μM CFSE- Carboxyfluorescein succinimidyl ester for 30 minutes at 22°C in shaking conditions in dark). After 30 minutes, the cells were washed thrice with phosphate buffer and mixed with unlabeled cells in 1:49 ratio (CFSE-AX2: AX2). The slugs formed from a mixed population were transferred to a non-nutrient agar plate with or without 5 mM caffeine. Soon after the slug transfer, the movement of CFSE labeled cells was monitored by taking pictures using a fluorescence microscope at the indicated time point. Arrow highlights the cell movement within the slug. Scale bar = 200 μm.