Figure 3

Expression of OSR1 and Osr2A leads to an increase of pronephros size in Xenopus laevis larvae. (A) Enlargement of pronephros: Dorsal view of stage 45 larvae [36] injected with 200 pg OSR1 mRNA and whole-mount immunostained with antibodies 3G8 and 4A6 [37]. The injected side is marked by an arrow. Bar = 200 μm (B) Ectopic kidney cell patches (marked by arrow heads): Lateral view of a stage 45 larvae [36] expressing OSR1 and prepared as in A. Bar = 200 μm (C) Pronephros size on injected versus control side in OSR1 or Osr2A injected embryos was determined in lateral views by measuring the area through the widest part of the immunostained pronephros. The Student's t-test was used to score significant differences. The p-value is given by comparing embryos injected with gene of interest plus GFP mRNA as marker with those injected with GFP mRNA taken from previous data [23]. _*** denotes p-value ≤ 0.001. The vertical line in the middle represents the mean and the small bars the standard deviation. N is the number of animals investigated.