Figure 6

Transcriptional Regulation of Zebrafish TαC. A. Schematic showing the location of PRE-1 and other upstream promoter regions known to regulate zebrafish TαC transcription. A green bar indicates the minimal cone-specific promoter and PRE-1 is represented by a red square. Upstream enhancer regions are indicated by red and orange rectangles, and CPRE-1 by a blue box. The region containing pineal enhancer(s) and CPRE-1 -dependent regions are indicated by a black rectangle. ATG is the translation start site and TSS is the predicted transcription start site. B. Schematic of the DNA bases in PRE-1 critical for complex formation with nuclear protein or for enhancement of reporter activity. The size of each letter is proportional to the importance of the nucleotide in in vitro EMSA and in vivo reporter assays. The alignment of critical PRE-1 residues to consensus E-box and Otx sites and to PRE-1-rho is also shown.