Figure 4

PRE-1 is not required for ~2.5 kb TαC promoter activity in vivo. A. Schematic of the 2521 bp TαC wildtype and PRE-1 deletion constructs and graph of their activity in transient transgenics at 5 dpf. Deletion of PRE-1 has no significant effect on the activity levels of the fragment. The number of transgenic fish scored for each construct is indicated at the right of the histograms. A red box highlights the PRE-1 element. B. Schematic showing the location of PRE-1-like sequences in 10 kb promoter fragments of zebrafish photoreceptor and housekeeping genes with common gene name to the right and gene symbol in brackets. Bioinformatic alignments reveal several PRE-1-like sequences in the ~2.5 kb TαC promoter suggesting redundancy. PRE-1-like sequences are also located in promoter fragments of housekeeping genes. However, the highest sequence similarity was found in the proximal rhodopsin promoter. C. Clustal W pairwise alignment of PRE-1 and PRE-1-rho with mismatched sequences highlighted in boxes.