Figure 2

Hypoxia exposure for three days induces embryonic growth retardation. (A) Embryos exposed to hypoxia for 2 days in utero from E8.5-10.5 were not growth retarded compared to room air controls, but embryos exposed to hypoxia in utero from E10-12.5 exhibited significant growth retardation. Embryos exposed to hypoxia for 3 days demonstrated an even greater amount of growth retardation compared to controls. (B-E) Representative pictures of the different treatment and genotype groups after 3 days are shown. Normoxic embryos both (B) Normox/Flox and (C) Normox/Cre displayed normal morphology and growth. The hypoxic embryos (D) Hypox/Flox and (E) Hypox/Cre were significantly growth retarded, however there was no difference between Hypox/Flox and Hypox/Cre embryos. Cre/+ embryos have A1AR deleted from cardiomyocytes. *, P ≤ 0.01; **, P ≤ 0.001. Scale bar is 1 mm.