Figure 3

Migrating cells travel same pathways as neural crest cells. A and B: Thick section of a DiI (red) and DAPI (blue) labeled snake embryo (Embryo 2 incubated for 12 hrs) on the second coil showed cells that migrated along the ventromedial pathway and settled by the dorsal aorta (D, A). Insert in B shows cells in more detail by the aorta. There was also a single DiI cell migrating sub-ectodermally (arrow in A, B). C and D show DiI (red) and DAPI (blue) labeled cells migrating on the most rostral portion of the somite (arrows in D point to area of the insert in D at higher magnification). This embryo (3 days POP) was labeled with DiI and incubated for 12 hrs at 37°C. N: notochord, DM: dermomyotome, NT: neural tube. R is for rostral; C is for caudal orientation in the embryo coils.