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Figure 5 | BMC Developmental Biology

Figure 5

From: Developmental cues and persistent neurogenic potential within an in vitro neural niche

Figure 5

Fluorescent antibody labelling indicates apoptotic cells in Day 14 in vitro NSC niche culture. A and B, TUNEL (shown in red) indicates apoptotic cells within the culture are found at the outer edge of aggregates and in the area between aggregates. Neurafilament (NF) (shown in green) labeling suggests that the apoptotic cells are localized among mature cells of the culture. C, Activated caspase-3 (shown in red) labeling further indicates apoptotic cells in areas between aggregates. β-III tubulin+ early neurons (in green) are indicated at the edge of the aggregate, within inter-aggregate processes and in the space between aggregates. D, Two cells expressing activated caspase-3 are shown along a neuronal process (labeled in green) between aggregates. E-H, Successive micrographs indicate a cell (arrow in H) that is positive for caspase-3 and β-III tubulin, indicating an apoptotic neuron. DAPI staining, shown in blue in all panels indicates nuclei. The scale bar in A is 50 μm and applies to A and B. Scale bar in C is 20 μm and applies to C-H.

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