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Figure 1 | BMC Developmental Biology

Figure 1

From: Developmental cues and persistent neurogenic potential within an in vitro neural niche

Figure 1

Scanning and Transmission Electron Microscopy (SEM and TEM) of the Day 14 in vitro NSC niche. A, SEM micrograph shows multicellular aggregates (arrowhead) and processes (arrow) between aggregates. Scale bar is 100 μm. B, Higher magnification of an aggregate shows many processes attached to a single aggregate. Scale bar is 10 microns. C, Cells of the aggregate shown in B include those with intact (arrow) and apoptotic (arrowhead) appearance. Scale bar is 3 μm. D, Cells (arrow) can be seen attached to processes (arrowheads). Scale bar is 3 μm. E, Magnification of D reveals the presence of varicosities (arrowheads) and blebs (arrows). Scale bar is 1 micron. F, Magnification of D including a cell (arrowhead) attached to the process. A very small process (arrow) is present on top of the migratory cell. Scale bar is 2 μm. G, TEM micrograph of a cell from the edge of an aggregate shows microvilli (arrows) and intracellular organelles. Scale bar is 0.5 μm. H, TEM micrograph of a section from an aggregate suggests the presence of cell-types. Scale bar is 4 μm.

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