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Figure 6 | BMC Developmental Biology

Figure 6

From: The long noncoding RNA RNCR2 directs mouse retinal cell specification

Figure 6

Overexpression of RNCR2-IRES-GFP has dominant-negative effects in vivo. CAG-driven constructs encoding IRES-GFP or RNCR2-IRES-GFP were electroporated into P0 retina and harvested at either P7 or P21 and analyzed. (A-B) RNCR2-IRES-GFP expression results in a decrease in rhodopsin expression and an increase in the fraction of amacrine cells at P7 and P21, and an increase in the fraction of GFP-positive cells not stained by any marker tested at P21. *p < 0.02. (C-D) Analysis of electroporated retinas at P21 confirms the increase in amacrine cells (yellow arrowheads). RNCR2-IRES-GFP led to an increase in INL cells compared to IRES-GFP (B, inset).

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