Figure 3
From: Characterization of the Six1 homeobox gene in normal mammary gland morphogenesis
Six1 -related family members Six2 and Six4 are upregulated in Six1-/- mammary glands. (A) qRT-PCR analysis confirms that Six1 transcript is not present in Six1-/-mammary glands. (B) Six2 and (C) Six4 RNA is elevated in Six1-/- mammary glands. (D) Six5 transcript levels are not significantly different in Six1-/- mammary glands as compared to wild-type controls. Data (A-D) was obtained from three pooled samples for each genotype, consisting of mammary glands harvested from 5 mice at embryonic day 18.5, representing a total of 15 animals for each genotype. Graphs are representative of two independent experiments. Error bars denote mean ± sem. P-values were obtained using a paired 2-tailed student's t-Test.