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Figure 3 | BMC Developmental Biology

Figure 3

From: SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells

Figure 3

Inhibited endocrine and exocrine cell differentiation in Sel1lβgeo/βgeoembryos. (A-F) Immunohistological analysis of the developing pancreas of Sel1l+/+ and Sel1lβgeo/βgeoembryos at E11.5 (A-B) and E15.5 (C-F); the genotypes of the pancreatic sections are indicated as "+/+" and "-/-", respectively. The following antibodies were used: Pdx1 (A-D, green), glucagon (A-D, red), insulin (E-F, red) and amylase (E-F, green). (A-B) At E11.5, Glu+ cells were detected in the pancreatic epithelium of wild-type and Sel1lβgeo/βgeoembryos in equal numbers. (C-D) At E15.5, the number of glucagon+ cells was reduced in Sel1lβgeo/βgeoembryos as compared to wild-type control. (E-F) No amylase+ cells were detected and the number of insulin+ cells was significantly reduced in Sel1lβgeo/βgeoembryos. (G-I) Statistical analyses of the numbers of glucagon+ and insulin+ cells in wild-type and Sel1lβgeo/βgeoembryos. Data were from three wild-type and three Sel1lβgeo/βgeoembryos. At E11.5, no significant difference in the number of glucagon+ cells in wild-type and Sel1lβgeo/βgeoembryos was detected (G). The number of glucagon+ and insulin+ cells was markedly lower in Sel1lβgeo/βgeoembryos than in wild-type control embryos (H and I, respectively). Scale bar: 100 μm.

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