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Figure 1 | BMC Developmental Biology

Figure 1

From: Generation and characterization of a Tet-On (rtTA-M2) transgenic rat

Figure 1

pLenti-Rosa26-rtTA-M2 and pLenti-TetO 7 /CMV-EGFP lentiviral vectors. Replication-deficient lentiviral vectors feature a self-inactivating 3'UTR and a CMV enhancer replaces the U3 region of the 5'UTR. WRE: woodchuck hepatitis posttranscriptional regulatory element. A. Expression of rtTA-M2 is driven by the ubiquitous Rosa-26 promoter. B. Expression of EGFP is under control of a seven repeat Tet operator and CMV minimal promoter (TetO7/CMV). In the presence of doxycycline (yellow triangles), The rtTA-M2 protein (blue ovals) encoded by the construct in (A) binds the TetO7 operator and transactivates EGFP expression from the construct in (B).

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