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Figure 5 | BMC Developmental Biology

Figure 5

From: Analysis of chick (Gallus gallus) middle ear columella formation

Figure 5

ISH analysis during columella development. Transverse sections of paraffin embedded embryos showing the columella from E5-E10, 10-12 μm. (A, B) Sox9 is expressed in the otic capsule and columella condensations. (C) Expression remains strong at E7, with the labeled extracolumella at E8 (D), but dramatically downregulated, with weak mosaic expression at E9 (E). (F, G) Bmp2 is not expressed in the columella at E5/6. (H, I) Bmp2 briefly upregulates during chondrogenesis, with transcripts also detected in the perichondrium (H-J) (J) Expression continues to weaken, except for the perichondrium (arrowed). (K-M) Bmp4 has weak, widespread expression at E5, downregulating at E6 and undetectable at E7. (N-O) Low expression levels recur at E8/9. (P, Q) Col2 is not expressed in the columella condensation and only weakly expressed in the otic capsule at E5/6. (R) Col2 upregulates in the otic capsule, columella, and extracolumella at E7, strengthening at E8 (S), rapidly clearing at E9 (T). Ihh up regulates at E10 in the otic capsule and columella, but not the perichondrium (inset, p, arrowed).

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