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Figure 2 | BMC Developmental Biology

Figure 2

From: Analysis of chick (Gallus gallus) middle ear columella formation

Figure 2

Morphology of the ear-forming region. E7 Toluidine Blue section, 12 μm showing the relationship of otic capsule to columella and extracolumella elements. The section is diagonal to include columella and extracolumella, with the latter more posteriorly situated relative to the notochord than the columella. Chondrogenic tissue stains pink, with the otic capsule, columella and cartilage around the notochord all stained. The extracolumella condensation appears blue, indicating that chondrogenesis has not yet begun. Note the perichondrium surrounding the columella (arrowed), and footplate (asterisk), which are also blue. Abbreviations: cd-cochlear duct, eam-external auditory meatus, fp-footplate, ie-inner ear, nt-neural tube, n-notochord, oc-otic capsule, pe-pharyngeal endoderm, tm-tympanic membrane, tts-tubotympanic sulcus, v-blood vessels.

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