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Figure 9 | BMC Developmental Biology

Figure 9

From: Activated Ras alters lens and corneal development through induction of distinct downstream targets

Figure 9

Corneal differentiation in Ras transgenic mice. In situ hybridizations (A, B) and immunohistochemistry (C-J) were performed on nontransgenic (NT) and Pax6-Ras transgenic embryos to detect expression of Hes1 (A, B), Keratin 12 (Krt12) (C, D), Keratin 14 (Krt14) (E, F), 14-3-3σ (G, H) and Trp63 (I, J). For in situ hybridizations (A, B), dark-field images were overlaid on respective bright-field images and silver grains were colored red. For immunohistochemistry (C-J), antigen antibody complexes are in red and nuclei are stained blue with DAPI. Ras transgenic corneal epithelial cells show an expansion of the Hes1 expression domain (B) and reduced Krt12 expression (D). Abbreviations; C, corneal epithelium; Cj, conjunctival epithelium; le, lens epithelium; lf, lens fibers; nr, neural retina. The scale bar (I) represents 100 μm in panels A-F, I, J and 50 μm in panels G, H.

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