Figure 6
From: Activated Ras alters lens and corneal development through induction of distinct downstream targets
Erk1/2 phosphorylation in Ras transgenic eyes. Immunohistochemistry was performed on E11.5 (A, B) and E13.5 (C, D) Ras transgenic (B, D) and nontransgenic (NT) (A, C) embryos using an anti-phospho-Erk1/2 antibody. Antigen antibody complexes are in red and nuclei are stained blue with DAPI. Elevated phospho-Erk1/2 levels were detected in the Ras transgenic lens epithelial cells (Compare C and D, green arrowheads). White arrows in C and D point to the lens equatorial region where fiber differentiation is initiated. Abbreviations; C, corneal epithelium; le, lens epithelium; lf, lens fibers; nr, neural retina. The scale bar (C) represents 50 μm in panels A-D.